Involvement of Estrogen Receptor and Mitochondrial KATP Channels in Cardioprotective Effect of Remote Aortic Preconditioning in Isolated Rat Heart

The present study was designed to investigate the role of estrogen receptor and KATP channel in cardioprotective effect remote aortic preconditioning rat heart. Remote is a phenomenon which brief episodes ischemia reperfusion organs protect target organ against sustained ischemia/ induced injury. Protective effects are well established heart, but their mechanisms still remain be elucidated. rats produced by introducing four reperfusion, each comprising 5 min occlusion through abdominal aorta. Perfused heart isolated subjected global for 30 followed 120 min. degree cardiac injury assessed analysing level lactate dehydrogenase, creatin kinase, reactive oxygen species nitrite coronary effluent. Myocardial infarct size also estimated macroscopically using tetrazolium tri chloride staining. Ethylinestradiol (10 μg/kg, orally) as agonist noted reproduce mediated cardioprotection ovariectomized rat. However, administration glibenclamide along with or prior ethylinestradiol abolished cardioprotection. Moreover, blocker, abolish daidzein caveolin inhibitor (0.2 mg/kg subcutaneous). significantly increased release nitric oxide restored attenuated preconditioning. These data provide evidence that activation involved preconditioning-mediated trough opening channels.